Coding

Part:BBa_K3225002:Design

Designed by: Yaran Zhao   Group: iGEM19_iBowu-China   (2019-10-16)


ExpR153, transcriptional factor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 530
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 720
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 252


Design Notes

The codon of ExpR was modified for E.coli.And BioBrick RFC10 assmbly method could be used.


Source

de-novo synthesis

References